4-nitrophenyl phosphate disodium salt hexahydrate reaction

  • pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9 ...

    pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9* ... 2.2 Incubate the reaction for 30 to 120 minutes at the desired temperature, protected from light.

  • p-Nitrophenylphosphate disodium salt hexahydrate | CAS 4264 ...

    Learn more about 4-Nitrophenylphosphate, Disodium Salt, Hexahydrate (pNPP-NA) and get the best prices in the life science marketplace at AG Scientific, Inc. We have over 20 years of experience with biochemical supplies.

  • PNPP, Disodium Salt | CAS 4264-83-9 | SCBT - Santa Cruz ...

    Buy this product (CAS 4264-83-9), an alkaline phosphatase substrate used in ELISA applications; absorbs light at 405nm, from Santa Cruz. Purity: ≥99%

  • 4-Nitrophenyl phosphate disodium salt hexahydrate powder ...

    4-Nitrophenyl phosphate disodium salt hexahydrate powder, BioReagent, suitable for cell culture, ≥97%; CAS Number: 333338-18-4; EC Number: 224-246-5; Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate; Linear Formula: C6H4NNa2O6P · 6H2O; find Sigma-Aldrich-N4645 MSDS, related peer ...

  • 4-Nitrophenyl phosphate disodium salt hexahydrate for enzyme ...

    4-nitrophenyl phosphate disodium salt hexahydrate has been used: • as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity • as substrate to assay acid phosphatase activity

  • 4-Nitrophenyl phosphate disodium salt hexahydrate ...

    4-Nitrophenyl phosphate disodium salt hexahydrate ≥99 %, p.a. Substrate for alkaline phosphatase, particularly for ELISA. p-Nitrophenyl phosphate, pNPP

  • 4-Nitrophenyl Phosphate | TCI America

    Product Information Volume : 100 mL Preservative : 0.02% Sodium azide Description This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears.

  • 4-Nitrophenyl phosphate disodium salt hexahydrate | Sigma-Aldrich

    4-Nitrophenyl phosphate disodium salt hexahydrate tablet Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate CAS Number 333338-18-4. Linear Formula O 2 NC 6 H 4 OP(O)(ONa) 2 · 6H 2 O . Molecular Weight 371.14 .

  • 4-Nitrophenyl Phosphate, Disodium Salt Hexahydrate - 4264-83 ...

    4-Nitrophenyl Phosphate, Disodium Salt Hexahydrate A colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction produces a soluble end product that is yellow in colour.

  • Safety Data Sheet - Northwest Missouri State University

    Product Number : N2765 Brand : Sigma REACH No. : A registration number is not available for this substance as the substance or its uses are exempted from registration, the annual tonnage does not require a registration or the registration is envisaged for a later registration deadline. CAS-No.

  • 4-Nitrophenyl Phosphate (pNPP) disodium salt ... - Roche

    4-Nitrophenyl Phosphate (pNPP) disodium salt, crystalline powder. For further processing only.

  • 4264-83-9 - 4-Nitrophenyl phosphate disodium salt hexahydrate ...

    4-Nitrophenyl phosphate disodium salt hexahydrate which acts as a chromogenic substrate for the determination of acid and alkaline phosphatases.

  • Phosphatase substrate powder | Sigma-Aldrich

    Synonym: 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate CAS Number 333338-18-4. Linear Formula O 2 NC 6 H 4 OP(O)(ONa) 2 · 6H 2 O . Molecular Weight 371.14 . EC Number 224-246-5. MDL number MFCD00066288. PubChem Substance ID 24898495

  • para-Nitrophenylphosphate - Wikipedia

    para-Nitrophenylphosphate ( pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP).

  • Disodium;(4-nitrophenyl) phosphate;hexahydrate ...

    Disodium;(4-nitrophenyl) phosphate;hexahydrate | C6H16NNa2O12P | CID 24802402 - structure, chemical names, physical and chemical properties, classification, patents ...

  • 4-Nitrophenyl phosphate, disodium salt hexahydrate

    4-Nitrophenyl phosphate, disodium salt hexahydrate PNPP, p-nitrophenyl phosphate, para nitrophenyl phosphate, PNP-phos · 2 Na MOLECULAR BIOLOGY GRADE Off white to light yellow crystalline powder Chromogenic substrate yielding a soluble yellow chromoph

  • 4-Nitrophenyl Phosphate |

    Product Information Volume : 100 mL Preservative : 0.02% Sodium azide Description This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears.

  • 4-Nitrophenyl phosphate disodium salt hexahydrate ...

    4-Nitrophenyl phosphate disodium salt hexahydrate | C6H16NNa2O12P | CID 2723710 - structure, chemical names, physical and chemical properties, classification, patents ...

  • 4-Nitrophenyl phosphate | C6H6NO6P - PubChem

    4-nitrophenyl phosphate is an aryl phosphate resulting from the mono-esterification of phosphoric acid with 4-nitrophenol. It has a role as a mouse metabolite. It has a role as a mouse metabolite. It derives from a 4-nitrophenol .

  • di-Sodium 4-nitrophenyl phosphate hexahydrate, Reagent Grade ...

    Disodium 4-nitrophenyl phosphate hexahydrate Preferred substrate for high sensitivity detection of alkaline phosphatase in EIA assays. Read at 405 nm. Soluble.Reaction Condition: 1 mg/ml in 10 mM Diethanolamine and 0.5 mM MgCl2, pH 9.5.

  • Disodium 4-nitrophenyl phosphate hexahydrate | VWR

    di-Sodium 4-nitrophenyl phosphate hexahydrate, Reagent Grade Supplier: VWR Description: Preferred substrate for high sensitivity detection of alkaline phosphatase in EIA assays.

  • 1. Identification Product Name 4-Nitrophenyl phosphate ...

    Product Name 4-Nitrophenyl phosphate disodium salt hexahydrate Cat No. : A12310 CAS-No 333338-18-4 Synonyms PNPP. Recommended Use Laboratory chemicals. Uses advised against Food, drug, pesticide or biocidal product use Details of the supplier of the safety data sheet Emergency Telephone Number

  • PNPP, Disodium Salt | CAS 4264-83-9 | SCBT - Santa Cruz ...

    The reaction produces a soluble end product that is yellow in color. Can be used to perform kinetic and endpoint determinations. Also used as a phosphorylating agent in nucleotide synthesis. Recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718). . 1.

  • pNPP-Na hexahydrate, Alkaline phosphatase ... - abcam.com

    I can confirm that ab146203 (pNPP-Na hexahydrate) is the correct substrate for Alkaline Phosphotase (coupled to an antibody). pNPP is hydrolyzed rapidly in the presence of Alkaline Phosphatase to p-nitrophenol and inorganic phosphate. The formed p-nitrophenol has an intense yellow color that is measured at 405 nm.

  • p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS ...

    p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS 4264-83-9 - Calbiochem p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM). - Find MSDS or SDS, a COA, data sheets and more information.

  • PNPP, Disodium Salt | CAS 4264-83-9 | SCBT - Santa Cruz ...

    The reaction produces a soluble end product that is yellow in color. Can be used to perform kinetic and endpoint determinations. Also used as a phosphorylating agent in nucleotide synthesis. Recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718). References. 1.

  • p-Nitrophenyl Phosphate Liquid Substrate System - Creative ...

    The reaction may be stopped with 3N NaOH and read at 405 nm. ... 4-Nitrophenyl phosphate disodium salt solution; pNPP ... ProductName 4-Nitrophenyl phosphate disodium ...

  • PNPP, Disodium Salt | CAS 4264-83-9 | SCBT - Santa Cruz ...

    PNPP, Disodium Salt (CAS 4264-83-9) ... Disodium 4-nitrophenyl phosphate hexahydrate; ... the reaction may be stopped with 3N NaOH and read at 405 nm.